Folate-conjugated immunoglobulin targets melanoma tumor cells for NK cell effector functions.

摘要: The folate receptor (FR) is overexpressed on the vascular side of cancerous cells including those breast, ovaries, testes, and cervix. We hypothesized that a folate-conjugated immunoglobulin (F-IgG) would bind to FR melanoma tumor target these for lysis by natural killer (NK) cells. Folate expression was confirmed in Mel-39 (human melanoma) cell line flow cytometry immunoblot analysis using KB oral epithelial) F01 as positive negative control, respectively. FR-positive FR-negative lines were treated with F-IgG or control G presence absence cytokines determine NK ability lyse lines. activation significantly upregulated Mel 39 increased following treatment compared at all effector : target (E : T) ratios (P<0.01). This trend further stimulation activating cytokine interleukin-12. production such interferon-gamma, macrophage inflammatory protein 1α, regulated normal T-cell expressed secreted (RANTES) also response costimulation interleukin-12 F-IgG-coated controls In contrast, did not had no significant effect production. research indicates potential use its induce an immune from against cells, which can be addition cytokines.