Suitability of the Composite Made of Multi Wall Carbon Nanotubes-Polyvinylpyrrolidone for Culturing Invertebrate Helix aspersa Neurons

摘要: Carbon nanotubes have been used as scaffolds for tissue engineering. However, the identification of these nanomaterials disperses in biological solutions and their direct interaction with nerve cells is still controversial. The aim this work to characterize topographic ultra-structural distribution composite made multi wall carbon nanotubes-polyvinylpyrrolidone (MWCNTs-PVP) deposited on Helix aspersa neurons over glass coverslip. Scanning Electron Microscopy (SEM) Confocal (CM) studies were done analyze properties such MWCNTs-PVP composite. cerebral ganglion was treated incubated MWCNTs-PVP, fixing it paraformaldehyde at 4% observed SEM CM. Although not labeled or stained fluorescent compounds, cells, by confocal microscope reflection mode. In studies, that attached surface neurons. Moreover, CM possible observe neuronal membrane, crossing cell membrane getting into cytoplasm. These results support hypothesis interact can be useful addition, open new alternatives toxicological order elucidate cytotoxicity other excitable cells.