Actin plays a role in both changes in cell shape and gene-expression associated with Schwann cell myelination.

摘要: Schwann cell (SC) differentiation into a myelinating requires concurrent interactions with basal lamina and an axon destined for myelination. As SCs differentiate, they undergo progressive morphological changes initiate myelin-specific gene expression. We find that disrupting actin polymerization cytochalasin D (CD) inhibits myelination of SC/neuron co-cultures. Basal is present, neurons are healthy, the inhibition reversible. Electron microscopic analysis reveals plays role at two stages SC differentiation. At 0.75–1.0 μg/ml CD, do not differentiate appear as “rounded” cells in contact axons. This morphology consistent disruption filaments shape changes. However, 0.25 partially differentiate; elongate segregate axons but generally fail to form one-to-one relationships spiral around axon. In situ hybridizations reveal CD-treated cultures express mRNAs encoding proteins 2′,3′-cyclic nucleotide phosphodiesterase (CNP), myelin-associated glycoprotein (MAG), P0. Our results suggest lower CD dose, commence evidenced by unable elaborate myelin lamellae because lack mRNAs. propose F-actin influences expression SCs.