Bacillus thuringiensis HD-1 Cry- : development of a safe, non-insecticidal simulant for Bacillus anthracis.

摘要: Aims A representative simulant for spores of Bacillus anthracis is needed field testing. thuringiensis gaining recognition as a suitable organism. A strain that does not form the insecticidal, parasporal crystals are characteristic this species more accurate physical B. anthracis spores. We developed noninsecticidal derivatives two isolates B. thuringiensis HD-1. Methods and Results Two plasmid-cured HD-1, unable to make crystal toxins (‘Cry−’), were isolated. These existing Cry− strain, Al Hakam, probed with PCR assays against known insecticidal genes cry, vip cyt. Their genomic DNA was sequenced demonstrate lack genes. This confirmed by bioassays number invertebrate species. Real-time identify HD-1 an effective differential selective medium assessed. Conclusions All three devoid determinants. The can easily be recovered from soil identified some selectivity. Significance Impact Study The represent accurate, nongenetically manipulated simulants excellent human environmental safety records.