Magnetic solid phase enzyme-immunoassay
作者： Jean-Luc Guesdon , Stratis Avrameas
DOI: 10.1016/0019-2791(77)90170-7
关键词:
摘要: Abstract A sandwich non-competitive enzyme-immunoassay procedure using antigen or antibody cavalently linked to magnetic polyacrylamide-agarose beads has been developed. rack was used separate the from liquid phase and therefore time-consuming multiple centrifugation avoided. Comparative studies various conjugates of peroxidase, glucose oxidase alkaline phosphate with antibodies were performed. In regard accuracy reproducibility enzyme immunoassay, best results obtained phosphatase labelled antibodies. The allowed measurement following lowest quantities proteins: 8 ng IgG, 40 IgA, 70 IgM, 1 i.u IgE 20 specific against BSA.
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