Bacterial Metabolites Produced Under Iron Limitation Kill Pinewood Nematode and Attract Caenorhabditis elegans

摘要: Pine Wilt Disease (PWD) is caused by Bursaphelenchus xylophilus, the pinewood nematode, and affects several species of pine trees worldwide. The ecosystem Pinus pinaster was investigated as a source bacteria producing metabolites affecting this ecosystem: P. target-plant, nematode disease effector its insect-vector shuttle. For example, metals metal-carrying compounds contribute to complex tree-ecosystems. This work aimed detect novel secondary like metallophores related molecules produced under iron limitation PWD-associated test their activity on nematodes. After screening 357 bacterial strains from Portugal United States, two promising metallophore-producing Erwinia sp. A41C3 Rouxiella Arv20#4.1 were chosen in more detail. genomes these sequenced, analyzed, used genetic potential for metabolite production. A combinatorial approach liquid chromatography-coupled tandem mass spectrometry (LC-MS) linked molecular networking describe compounds. Two major detected HPLC analyses described. One fraction strain showed be hydroxamate-type siderophore with higher affinity chelation Cu. highest metal catecholate-type Fe. LC-MS allowed identification desferrioxamines Arv20#4.1, special desferrioxamine E, but no hit obtained case which might indicate that it something new. Bacteria culture supernatants ability attract C. elegans. fractions those supernatant-extracts A41C3, enriched such siderophores, able kill nematode. These results suggest secreted have biocontrol B. xylophilus management Disease.