Suppression of lymphokine-activated killer cell generation by tumor-infiltrating lymphocytes

摘要: Abstract A functional analysis of tumor-infiltrating lymphocytes (TILs) from renal cell carcinoma (RCC) and malignant melanoma was performed. TILs were expanded in recombinant interleukin-2 (50 U/ml) Iscoves medium. Phenotypic (cytolytic vs regulatory) analyses carried out with the fresh TIL populations after 4 weeks culture. Only one population an RCC case (out six cases studied) CD8+ demonstrated MHC class I-restricted tumor-specific cytotoxicity against autologous target. other five became predominantly CD4+ they neither killed respective tumor cells nor NK-sensitive target K-562 cells. When studied for functions, two found to suppress lymphokine-activated killer response by peripheral blood (PBL) coculture. Of these two, a (MJ TIL) used study cellular molecular mechanisms suppression. The MJ synthesized supernatant factor that blocked activation resting PBL as measured induction high-affinity IL-2 receptor (IL-2R) when stimulated phytohemagglutinin but did not down-regulate fully expressed IL-2R on activated T suppression result necrosis factor-α β or transforming growth factor-β cytokines detected cell-free also suppressed IL-2-mediated enhancement natural (NK) without demonstrating direct toxic effect NK Thus, are adoptive immunocytotherapy, it may be useful characterize them functionally, vitro .