Peculiarities of host cholesterol transport to the unique intracellular vacuole containing Toxoplasma.
作者： Alfica Sehgal , Silvana Bettiol , Marc Pypaert , Markus R. Wenk , Achim Kaasch
DOI: 10.1111/J.1600-0854.2005.00348.X
关键词:
摘要: The intracellular protozoan Toxoplasma gondii is auxotrophic for low-density lipoprotein (LDL)-derived cholesterol (C). We previously showed that T. scavenges this essential lipid from host endolysosomal compartments and C delivery to the parasitophorous vacuole (PV) does not require transit through Golgi or endoplasmic reticulum. In study, we explore itinerary of endolysosomes PV. Labeled incorporated into LDL rapidly detected in intravacuolar parasites partially esterified by parasites. contrast diverse mammalian organelles, post-endolysosomal transfer PV involve plasma membrane as an intermediate. Nevertheless, accessible extracellular sterol acceptors, suggesting trafficking membrane. movement requires temperatures permissive vesicular transport, metabolic energy functional microtubules. Host caveolae vesicles carrier protein-2 do participate process. Proteolytic treatment purified free abolishes acquisition Altogether, these results support a transport system PV, requirement parasite proteins gondii.
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