Molecular evolution of the vertebrate blood coagulation network.

摘要: In mammalian blood coagulation 5 proteases, factor VII (FVII), IX (FIX), X (FX), protein C (PC) and prothrombin act with two cofactors V VIII to control the generation of fibrin. Biochemical evidence molecular cloning data have previously indicated that involving tissue factor, fibrinogen is present in all vertebrates. Using degenerate RT-PCR we isolated characterized novel cDNAs sequence identity serine proteases from chicken puffer fish (Fugu rubripes). Sequence alignments, phylogenetic comparative analysis support existence Gla-EGF1-EGF2-SP domain proteasesFVII, FIX, FX, PC A1-A2-B-A3-C1-C2 FV FVIII these species. These results strongly suggest network jawed vertebrates evolved before divergence tetrapods teleosts over 430 million years ago; vertebrate may benefited rounds gene or whole genome duplication. Sequences identified Fugu coding for additional FVII-like, FIX-like PC-like sequences possibility further tandem large-scale duplications teleosts. Comparative analyses amino acid residues active site region new as yet unknown functions.