Dry-reagent disposable dipstick test for visual screening of seven leukemia-related chromosomal translocations

摘要: We report the first dry-reagent, disposable, dipstick test for molecular screening of seven chromosomal translocations associated with acute and chronic leukemia. The assay offers about 10 times higher detectability than agarose gel electrophoresis and, contrary to electrophoresis, allows confirmation sequence polymerase chain reaction (PCR) product by hybridization within a few minutes without need instrumentation. Biotinylated amplified DNA is hybridized dA-tailed probe applied strip, which contains oligo(dT)-conjugated gold nanoparticles in dry form. Upon immersion strip appropriate buffer, solution migrates hybrids are captured immobilized streptavidin at zone generating characteristic red line. excess oligo(dA) strands control producing second studied the: t(9;22)(q34;q11), t(15;17)(q22;q21), t(11;17)(q23;q21), t(5;17)(q32;q21), t(11;17)(q13;q21), t(8,21)(q22;q22) inv(16)(p13;q22) that generate BCR-ABL, PML-RARa, PLZF-RARa, NPM-RARa, NuMA-RARa, AML1-ETO CBFβ-MYH11 fusion genes, respectively. A single K562 cell was detectable amidst 106 normal leukocytes. developed actin, as reference gene. probes can be used identification transcripts involved translocation.