Visual screening for JAK2V617F mutation by a disposable dipstick

摘要: During the last 5 years, it was discovered that JAK2V617F somatic mutation is present in virtually all patients with polycythemia vera and a large proportion of essential thrombocythemia, primary myelofibrosis, refractory anemia ring sideroblasts thrombocytosis. As result, incorporated as new clonal marker 2008 revision WHO diagnostic criteria. Current methods for JAK2 genotyping include direct sequencing, pyrosequencing, allele-specific PCR electrophoresis, restriction fragment length polymorphism, real-time PCR, DNA-melting curve analysis, denaturing HPLC. Some these are labor intensive time consuming, while others require specialized costly equipment reagents. We report method detection allele by naked eye using dipstick test dry-reagent format. The comprises triprimer combined visual products within minutes test. Specialized instrumentation not involved. requirements highly qualified technical personnel minimized. Because reagents exist dry form on dipstick, there no need multiple pipetting incubation steps.