Disruption of arginase II alters prostate tumor formation in TRAMP mice.

摘要: BACKGROUND Arginase II (AII) is involved in the polyamine synthetic pathway, and elevated levels of expression have been found a high proportion prostate cancer samples patients. However, biological function arginase still remains to be elucidated. In this study, we utilized TRAMP mouse model better understand contribution AII on tumor development. METHODS AII was determined prostates from mice at 23 weeks age by real-time RT-PCR Western blot analysis. Additionally, disrupted crossbreeding knockout (AII KO) with order generate TRAMP/AII KO line. each group, genito-urinary (GU) tract weights were pathological evaluation tumors completed. RESULTS AII only detectable those without presence macroscopic tumors; it also absent TRAMP-C2 cell line, which characteristic an advanced tumor. Assessment GU revealed larger average compared mice. greater percentage more pathology group cohort. CONCLUSIONS Based these results, deficiency seems accelerate progression, leading overall stage These findings support possibility that prostatic could potentially useful marker disease progression. Prostate 68: 1561–1569, 2008. © 2008 Wiley-Liss, Inc.