Cyclophilin A is present in rat germ cells and is associated with spermatocyte apoptosis

摘要: Recent investigations in our laboratory revealed divalent cation-dependent endonuclease activity testes from 2-methoxyethanol-treated rats, which was able to cleave substrate DNA into a pattern of fragmentation consisting 180-200 base pairs. Further studies were undertaken characterize the active nuclease. F344 rats treated with 2-methoxyethanol, glycol ether that causes death pachytene spermatocytes juvenile and adult rats. The nuclease found nuclear extract animals, but not controls. A radioactive gel assay, detects degradation loss 32 P-labeled DNA-containing polyacrylamide gel, localized band 18 kDa. This dependent on calcium inhibited by both zinc aurintricarboxylic acid. Amino acid sequence data showed this protein identical cyclophilin A. Immunohistochemistry using antibodies against specific staining spermatocytes, spermatids, interstitial cells, Sertoli cell nuclei. Cyclophilin present control rat stage-dependent manner, stage-VIII-XIV seminiferous tubules most heavily stained. These demonstrate testis germ cells contain relatively high levels whose is associated spermatocyte apoptosis induced 2-methoxyethanol.