Fluorometric microbore amino acid analyzer: The construction of an inexpensive, highly sensitive instrument using o-phthalaldehyde as a detection agent
作者： How-Ming Lee , M.Diane Forde , Mei C. Lee , Doris J. Bucher
DOI: 10.1016/0003-2697(79)90585-2
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摘要: Abstract The construction of a highly sensitive microbore amino acid analyzer is described. It based on standard column chromatographic separation technique with fluorometric detection utilizing o -phthalaldehyde. This has several desirable features: low pressure, high sensitivity, and easy maintenance. Good precision at level 10 pmol obtained as little 0.2 μg protein been hydrolyzed for composition analysis. incorporates the use single-column method constant molarity buffers to shorten analysis time. fully automatic capable analyzing 130 samples within 7 days attention required only reloading 20 while instrument in operation. Amino determination peak area height discussed.
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