Using gut content immunoassays to evaluate predaceous biological control agents: a case study
作者： R. Hagler
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摘要: ABSTRACT Pest-specific monoclonal antibodies (MAbs) can be used to identifykey predaceous biological control agents. A MAb library was usedto (i) identify key indigenous predators of the sweetpotato whitefly, Bemisia tabaci (Gennadius) and pink bollworm, Pectinophora gossypiella (Saunders) (ii) monitor efficacy an augmentativepredator release targeted for these two pests.The gut contents over 10000 individual indigenousto Arizona cotton fields were examined presence whiteflyand bollworm egg antigens by enzyme-linkedimmunosorbentassay (ELISA). Five predator species with frequent positive assaysfor whitefly proteins (% positive): Collops vittatus (Say) (55%), Geocoris spp. nymphs (39%), Orius tristicolor (White) (38%) Hippodamia convergens Guerin-Meneville (33%). The most frequentpink Lygus hesperus Knight (adults 30%,nymphs 20%). 1. also th~ thehighest percentage individuals scoring both pest eggantigens (12%).In a separate experiment, we compared commercially reared
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