Effect of very low molecular weight henarin-derived oligosaccharides on lipoprotein lipase release in rabbits

摘要: Oligosaccharide fragments of heparin were prepared using flavobacterial heparinase. Following sizing, these oligosaccharide fractions administered (i.v.) to rabbits and examined for their ability release lipoprotein lipase. The decasaccharides (dp = 10, Mr avg 2,800) the smallest oligosaccharides which resulted in substantial lipase release. plasma levels obtained with comparable low molecular weight one-third those when was at an equivalent dose. peak concentration observed 10 min following heparinization fell off rapidly over 60-min time course. activity paralleled vivo pharmacokinetics decasaccharide sample as determined by monitoring anti-Factor Xa activity. No activation purified bovine milk or detectable concentrations studied, indicating that increase circulating lipolytic due entirely Lipoprotein accounted a major portion released hepatic triglyceride representing remainder sized characterized regards its structure anticoagulant exhibited reduced possibly making it better drug candidate treatment atherosclerosis.