Elimination of high affinity heparin fractions and their anticoagulant and lipase activity

摘要: High and low affinity heparin (HA LA heparin) were prepared from commercial by chromatography to insolubilized antithrombin III. HA was radiolabeled with 35S subdivided gel into high molecular weight (HMW, average 17,000– 26,000 daltons), intermediate (MMW, 12,000– 13,000 (LMW, 5,000–7,000 very (VLMW, 4,600 daltons) fractions. The kinetics of lipolytic anticoagulant activity protein-bound radioactivity studied after intravenous injection these failed induce but released the hepatic triglyceride lipase (H-TGL) lipoprotein (LPL) activities normally. VLMW LMW release both enzymes did not measurable activated partial thromboplastin time (APTT). A powerful effect found in anti-Xa assay, which disappeared according a continuously concave curve semilogarithmic plots, elimination rates similar those radiolabel. other preparations induced all measured. Heparin estimated two assays following convex curve, preceded rapid initial phase plots. disappearance plasma factor Xa inactivation similar. Peak values attained rapidly. H- TGL activity, as well LPL curves radioactivity. On basis kinetics, we suggest that, administration heparin, present are complexed analogous heparin-antithrombin III complex. Finally, kinetic data indicate that is determined part complexes, probably removal free heparin.