Microarray Analyses of Genes Differentially Expressed by Diet (Black Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in Rats.

摘要: We previously demonstrated that black bean (BB) and soy flour (SF)-based diets inhibit azoxymethane (AOM)-induced colon cancer. The objective of this study was to identify genes altered by carcinogen treatment in normal-appearing colonic mucosa those attenuated feeding. Ninety-five male F344 rats were fed control (AIN) upon arrival. At 4 5 weeks, injected with AOM (15 mg/kg) or saline one week later administered an AIN, BB-, SF-based diet. Rats sacrificed after 31 microarrays conducted on RNA isolated from the distal mucosa. induced a number involved immunity, including several MHC II-associated antigens innate defense (RatNP-3, Lyz2, Pla2g2a). BB- SF-fed exhibited higher expression energy metabolism water sodium absorption lower Pla2g2a, Tlr4, Dmbt1) cell cycle-associated (Cdc2, Ccnb1, Top2a) genes. Genes extracellular matrix (Col1a1, Fn1) immunity Pla2g2a) all diets, but extent bean-fed animals. This profile suggests beans carcinogenesis modulating cellular kinetics reducing inflammation, potentially preserving mucosal barrier function.