Differential response of human naive and memory/effector T cells to dendritic cells infected by respiratory syncytial virus.

摘要: In vitro studies have contributed substantially to the understanding of immunopathology respiratory syncytial virus (RSV)-mediated disease. present study we compared effect RSV-infected dendritic cells on time-course primary and memory/effector T cell response in vitro. Cultures with uninfected known elicit helper 2 (Th2) responses polyinosinic-polycytidylic acid (poly-IC)-stimulated Th1 served as controls. At day 1 after stimulation there was a high proportion interleukin (IL)-2 tumour necrosis factor (TNF)-alpha-producing no difference number producing well concentration secreted cytokines between control cultures. However, up 3 generation IFN-gamma reduced markedly. addition, proliferation RSV 7 RSV-treated cultures showed preponderance IL-4 generation. days 21-24, three rounds restimulation, matured under influence were still not fully polarized but contrast displayed predominance cytokines. Contact HEp-2 inhibited cells; memory effector less sensitive contact inhibition than naive cells. stimulated rearrangement cortical actin more effectively summary, shown that infection has distinct modulatory pronounced response.