Characterization of a primitive erythropoietic progenitor found in mouse marrow before and after several weeks in culture.

摘要: Abstract The properties of a primitive mouse erythropoietic progenitor cell capable macroscopic erythroid burst formation in methyl cellulose or agar have beert studied In assays normal marrow. bursts that can achieve size (approxi-mately 106 cells/burst) constitute minor Proportion (30 %) all present after 2-wk incubation. When marrow is first cultured flasks containing serum but no added erythropoietin (Ep), within 2 wk progenitors become the predominant type, and 1 isolated erythroblast Clusters (CFU-E) small rapidly maturing (day-3 BFU-E) are longer detectable. processes compositions from both sources identical. Macroscopic late maturation pattern; many, hemoglobinizing erythro-blasts not found until 12-14 days plating. greatly enhanced when pokeweed-mito-gen-stimulated spleen-cell-conditioned medium (PWM-SCCM) to assay culture, this reduces Ep requirement. However, even pre-sence PWM-SCCM requirement high; it equals exceeds concentra-tion required for late-maturing microscopic bursts. Morphologic analysis 185 individual indicated almost 90% contained megakaryo-cytes as well progeny. replating experiments, types precursors were detected vary-ing numbers 12-day-old One these actually gave rise another two bursts, each megakaryocytes erythroblasts. This confirms extensive proliferative bipotent differentiation capacities suggests they may also self-renewal capabilities. Time course studies number nonadherent fraction flask cultures showed be regularly significantly increased wk. An association between recovery CFU-S was ed. These results indicate provide simple end point identifying uncommit-ted hemopoietic adult availability culture System permits production restricts its subsequent differentiation, should useful future early events.