CfPDIP1, a novel secreted protein of Colletotrichum falcatum, elicits defense responses in sugarcane and triggers hypersensitive response in tobacco

摘要: Colletotrichum falcatum, a hemibiotrophic fungal pathogen, causes one of the major devastating diseases sugarcane—red rot. C. falcatum secretes plethora molecular signatures that might play crucial role during its interaction with sugarcane. Here, we report purification and characterization novel secreted protein elicits defense responses in sugarcane triggers hypersensitive response (HR) tobacco. The purified from culture filtrate was identified by MALDI TOF/TOF MS designated as plant defense-inducing 1 (CfPDIP1). Temporal transcriptional profiling showed level CfPDIP1 expression greater incompatible than compatible until 120 h post-inoculation (hpi). EffectorP, an silico tool, has predicted potential effector. Functional full length two other domain deletional variants (CfPDIP1ΔN1-21 CfPDIP1ΔN1-45) recombinant proteins indicated CfPDIP1ΔN1-21 variant elicited rapid alkalinization induced relatively higher production hydrogen peroxide (H2O2) suspension culture. However, Nicotiana tabacum, all three forms triggered HR along induction H2O2 callose deposition. Further using detached leaf bioassay revealed foliar priming CfPDIP1∆1-21 suppressed extent lesion development, even though co-infiltration on unprimed leaves increased development control. Besides, systemic defense-related genes concomitant reduction pathogen biomass thereby suppression red rot severity Comprehensively, results have suggested protein, CfPDIP1, to trigger multitude tobacco upon plant-pathogen interactions.