Morphologic correlations with fluorophotometric data from monkey eyes with anterior uveitis.

摘要: Acute anterior uveitis was induced in monkeys by unilateral intravitreal injection of 1.0 ng Escherichia coli endotoxin. Twenty-four hours later, each animal received an intravenous 250 mg/kg body weight fluoresceinated horseradish peroxidase (F-HRP), and fluorophotometric measurements were taken for 90 min. The animals killed, both eyes processed HRP demonstration. In the chamber aqueous humor normal control eyes, F-HRP concentrations less than 0.002 mg/ml at concentration elevated consistently endotoxin-injected eyes; however, magnitude effect varied. By fluorophotometry, inflamed fell into two distinct groups. At min, most had 0.014-0.06 mg/ml, although others 0.39 mg/ml. latter group, appreciably shorter latency observed between time tracer its detection chamber. Aqueous protein concentrations, highest F-HRP-permeable followed more a continuum their distribution identified clearly subpopulations seen fluorophotometry. Normal no leakage across either ciliary epithelial or iris vascular endothelial barriers. All epithelium, but subpopulation with latencies higher fluorophotometry also leakage. Invest Ophthalmol Vis Sci 33:1642-1649, 1992