Agro Waste Utilization for Cost-Effective Production of l-Asparaginase by Pseudomonas plecoglossicida RS1 with Anticancer and Acrylamide Mitigation Potential.

摘要: Agricultural wastes such as the peels of onion and garlic were used a supplement along with l-asparagine for very first time to produce increased yield l-asparaginase by Pseudomonas plecoglossicida RS1. Statistical optimization strategies response surface methodology generate medium composition containing extracts 0.9 (v/v) peel waste 0.5% 0.2% (w/w) l-asparagine, which yielded twofold increase in enzyme activity compared unsupplemented minimal (M-9) medium. The presence content extract was confirmed high-performance liquid chromatography. Further, purified homogeneity, identity matrix-assisted laser desorption ionization time-of-flight analysis. application therapeutic studied HeLa cells showed p53-mediated G2 cell cycle arrest. Moreover, effective...