Developmental response of Spodoptera litura Fab. to treatments of crude volatile oil from Piper betle L. and evaluation of toxicity to earthworm, Eudrilus eugeniae Kinb.

摘要: Evaluations of biological effects (Pb-CVO) the crude volatile oil Piper betle leaves on tobacco cutworm Spodoptera litura were conducted. Pb-CVO was subjected to GC-MS analysis and twenty vital compounds isolated from betel leaf oil. tested at four different concentrations (0.25, 0.5, 1.0 1.5%) against S. litura. The treated insects exhibited dose depended mortality. mortality rate significantly higher 1.5% Pb-CVO. LC50 (Lethal concentration) observed 0.48% Larval pupal durations increased in all treatment 0.3, 0.4 0.5%) whereas, weight decreased compared control. Adult longevity S. litura reduced treatments but predominantly 0.5% Correspondingly, mean fecundity Histological studies larvae mid-gut profiles severely damaged showed abnormalities cells with 0.25 treatments. Earthworm toxicity illustrated that 0.1% chemical insecticides (monocrotophos cypermethrin) varied widely their contact toxicities 0.5 1.0% control both filter paper artificial soil test. These findings suggest essential significant inhibitors development caused behavioral changes Treatment these had no adverse effect earthworm populations.