The role of pre- and post-anaphase microtubules in the cytokinesis phase of the cell cycle.

摘要: Abstract The cytokinesis phase, or C of the cell cycle results in separation one into two daughter cells after completion mitosis. Although it is known that microtubules are required for proper positioning cytokinetic furrow [1,2], role pre-anaphase has not been clearly defined three key reasons. First, inducing microtubule depolymerization stabilization before onset anaphase blocks entry and via spindle checkpoint [3]. Second, organization changes rapidly at as mitotic kinase, Cdc2–cyclin B, inactivated [4]. Third, time between initiation very short, making difficult to unambiguously alter polymer levels cytokinesis, but inactivation checkpoint. Here, we have taken advantage discovery microinjection antibodies protein Mad2 (mitotic arrest deficient) prometaphase abrogates checkpoint, producing premature chromosome separation, segregation, normal [5,6]. To test were disassembled prophase cells, then injected with anti-Mad2 recorded through phase. show exit from mitosis absence triggered a 50 minute period cortical contractility was independent microtubules. Furthermore, upon reassembly during this contractile C-phase period, ∼30% underwent poleward movement, formed midzone complex, completed cytokinesis.