Single-stranded DNA-binding proteins as transcriptional activators
作者： Alita Miller , Xing Dai , Mieyoung Choi , M.Alexandra Glucksmann-Kuis , Lucia B. Rothman-Denes
DOI: 10.1016/S0076-6879(96)74004-1
关键词:
摘要: Publisher Summary This chapter describes the procedures for purification of N4 virion RNA polymerase and single-stranded DNA-binding protein, in vitro transcription assays, characterization DNA structure at early promoters, investigation interaction between EcoSSB promoter-containing, templates. resembles integration host factor (IHF) eukaryotic I activator UBF. These proteins bind a sequence-specific manner to bend upon binding, allowing contact transcriptional activators with respective machineries. Even though is not unusual properties promoters provide specificity process activation. In contrast, activity N4SSB required multifunctional protein that contains activation domain responsible direct E. coli polymerase.
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