Thermally-induced changes in chromatin of isolated nuclei and of intact cells as revealed by acridine orange staining
作者： Z. Darżynkiewicz , F. Traganos , T. Sharpless , M. Melamed
DOI: 10.1016/S0006-291X(74)80219-6
关键词:
摘要: Summary Thermal denaturation of DNA in situ is studied individual cells (or isolated nuclei) by staining with acridine orange and measuring fluorescence a rapid, automated flow-through cytofluorimeter. Acridine differentially stains native denatured prevents renaturation. Cells are suspensions, equilibrium the dye. Denaturation sensitive to ionic strength occurs at temperatures similar those reported for preparations soluble nucleo-histone. Histones appear stabilize against denaturation. There changes chromatin submelting as evidenced increased binding decreased light scattering. Present results differ from what was when formaldehyde used prevent It suspected that produces severe artifacts studies melting .
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