Comprehensive analysis of the p53 status in mucosal and cutaneous melanomas

摘要: The abrogation of the function “gatekeeper genome”, p53, is most prevalent molecular alteration in solid human tumors. Regarding melanomas involvement p53 alterations discussed controversially to date. In order evaluate status detail, primary tumors and metastases 63 sporadic cutaneous (CM) mucosal (MuM) were examined by immunohistochemistry sequence analysis entire coding region transcript, i.e., exons 2 11. addition, loss heterozygosity (LOH) allele-specific transcription (LOT) determined. Accumulation protein occurred CM MuM specimens (71% 58%, respectively). contrast, stabilizing mutations observed 14% no mutation was found specimens. Two aberrations located outside core domain. LOH detected 22% 58% MuM, LOT 25% genotype distribution at polymorphic codon 72 melanoma patients differed significantly from control subjects. calculation odds ratios (OR) 95% confidence intervals (CI) indicated an increased risk for developing individuals carrying Pro-coding allele. Altogether, aberrant expression appears be a common event both MuM. © 2005 Wiley-Liss, Inc.