摘要: In reference to our recent paper in PNAS (1), Rodriguez (2) raises an interesting point regarding the functionality of nuclear export sequences on ubiquitin-specific protease 12 (Usp12). To address this concern, we provide details sequence and a possible explanation discrepancy experimental observations. Garcia-Santisteban et al. (3) previously reported silico-based prediction approach identify signals (NESs), which they further validated experimentally using assay (4). This relies transient transfection NES-defective form Rev-GFP [pRev(1.4)GFP] into HeLa cells, accumulates … [↵][1]1Email: sanyal{at}hku.hk. [1]: #xref-corresp-1-1
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