Evidence that Heparin but Not Hirudin Reduces PAI-1 Expression in Cultured Human Endothelial Cells
作者： Josune Orbe , Ramón Montes , Natalia Zabalegui , Ana Pérez-Ruiz , José A Páramo
DOI: 10.1016/S0049-3848(98)00203-5
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摘要: Heparin and other antithrombotic drugs besides their anticoagulant action could have a profibrinolytic effect. We analyzed the effect of unfractionated heparin (UFH) hirudin on PAI-1 gene expression in human umbilical vein endothelial cells (HUVEC). Cells were stimulated with UFH (1 10 IU/ml) (20 100 TIU/ml). Samples obtained before 2, 6, 24 hours after stimulation. mRNA analysis was conducted by reverse transcription followed polymerase chain reaction, antigen determined ELISA. Addition (10 to HUVEC resulted decrease at 6 (40% reduction) (60% antigen. Hirudin, however, did not modify significantly nor inhibitor secretion. The addition or endotoxin-stimulated also reduced increased secretion (45%), whereas no be observed hirudin. Our results suggest that UFH, but hirudin, reducing might
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