Effects of Insulin-Like Growth Factor 1 on the In Vitro Development of Mouse Embryos after Cryopreservation

作者: E. A. Kizilova , S. Ya. Amstislavsky , E. Yu. Brusentsev , T. N. Igonina , S. V. Ranneva

DOI: 10.1134/S1062360421020028

关键词:

摘要: Cryopreservation of gametes and embryos is widely used for mammalian genome resource banking. Effects insulin-like growth factor-1 (IGF-1) on the in vitro development cleavage stage CD1 mouse after their cryopreservation were studied. The developed vivo up to 4–6-cell frozen a programmable freezer according standard protocol with 10% propylene glycol as cryoprotectant thereafter thawed cultured KSOM 48 h. percentage blastocyst culture medium supplemented IGF-1 (40 ng/mL) was higher compared controls (78.9 47.4%, respectively, p < 0.01). Moreover, blastocysts presence contained more cells (79.8 ± 3.9 58.6 3.6 cells, 0.001). However, index nuclear fragmentation did not differ between these groups. Experimental results presented here revealed stimulating effects preimplantation cryopreservation.

参考文章(17)
Patricia Bowman, Anne McLaren, Cleavage rate of mouse embryos in vivo and in vitro Development. ,vol. 24, pp. 203- 207 ,(1970) , 10.1242/DEV.24.1.203
Sergei Amstislavsky, Eugeny Brusentsev, Elena Kizilova, Tatyana Igonina, Tatyana Abramova, Irina Rozhkova, Embryo cryopreservation and in vitro culture of preimplantation embryos in Campbell's hamster (Phodopus campbelli). Theriogenology. ,vol. 83, pp. 1056- 1063 ,(2015) , 10.1016/J.THERIOGENOLOGY.2014.12.013
Kazuhiro Kawamura, Yuan Chen, Yimin Shu, Yuan Cheng, Jie Qiao, Barry Behr, Renee A. Reijo. Pera, Aaron J. W. Hsueh, Promotion of Human Early Embryonic Development and Blastocyst Outgrowth In Vitro Using Autocrine/Paracrine Growth Factors PLoS ONE. ,vol. 7, pp. e49328- ,(2012) , 10.1371/JOURNAL.PONE.0049328
Alan H. Handyside, Susan Hunter, Cell division and death in the mouse blastocyst before implantation. Roux's archives of developmental biology : the official organ of the EDBO. ,vol. 195, pp. 519- 526 ,(1986) , 10.1007/BF00375893
E. Yu. Brusentsev, T. N. Igonina, S. Ya. Amstislavsky, Traditional and modern approaches to culture of preimplantation mammalian embryos in vitro Russian Journal of Developmental Biology. ,vol. 45, pp. 53- 65 ,(2014) , 10.1134/S1062360414020039