Increasing the transient expression of GUS gene in Porphyra yezoensis by 18S rDNA targeted homologous recombination
作者： Hongquan Liu , Wengong Yu , Jixun Dai , Qianhong Gong , Kunfeng Yang
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摘要: In order to test whether 18S rDNA can influence positively GUS gene transient expression in the red alga Porphyra yezoensis, a targeting vector pQD-GUS was constructed containing portion of P. yezoensis and transformed it into same strain protoplasts. The results showed that protein activity increased markedly with compared parent pBS-GUS. It is suggested this system be used enhance exogenous genes transgenic yezoensis.
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