MicroRNA-197 Mediates the Overgrowth and Anti-Apoptotic Effects by Downregulating Insulin-Like Growth Factor-Binding Protein-3 During Nephroblastoma Tumorigenesis

摘要: MiR-197 is frequently upregulated to induce a series of oncogenic effects, which closely associated with poor survival and prognosis multiple malignancies. However, the roles miR-197 in tumorigenesis detailed molecular mechanism Wilms tumor (WT) have rarely been reported. This study aimed evaluate expression WT vivo potential effects on proliferation apoptosis SK-NEP-1 cells. A total 15 patients pathologically confirmed diagnosis paraneoplastic controls were enrolled. Real-time quantitative PCR (RT-qPCR) identified upregulation downregulation insulin-like growth factors binding protein 3 (IGFBP3) tissues comparison adjacent normal tissue (p < 0.001). CCK-8 flow cytometry assay found that inhibition caused significantly reduced along dramatically enhanced cells 0.01). IGFBP3 was overexpressed by pEGFP-C1-IGFBP3 plasmid transfection. Overexpression suppressed induced Further detected decreased mimics increased inhibitor compared mock Dual luciferase reporter revealed direct interaction between 3'-UTR site IGFBP3. Overall, above results indicated targeted overgrowth anti-apoptotic cells, could promote nephroblastoma tumorigenesis. Therefore, may be further assessed as target for treatment WT.