Aurora-B and Rho-kinase/ROCK, the two cleavage furrow kinases, independently regulate the progression of cytokinesis: possible existence of a novel cleavage furrow kinase phosphorylates ezrin/radixin/moesin (ERM).
作者： Tomoya Yokoyama , Hidemasa Goto , Ichiro Izawa , Hitoshi Mizutani , Masaki Inagaki
DOI: 10.1111/J.1365-2443.2005.00824.X
关键词:
摘要: Cytokinesis is regulated by several protein kinases, such as Aurora-B and Rho-kinase/ROCK. We have indicated that these two kinases are the cleavage furrow (CF) accumulate at phosphorylate intermediate filament (IF) proteins into daughter cells. It has been reported phosphorylates MgcRacGAP to functionally convert a RhoGAP during cytokinesis. Therefore, we investigated here relationship between Rho-kinase/ROCK in cytokinesis, using small interfering RNA (siRNA) technique. depletion did not alter furrow-specific localization of vice versa. Treatment or siRNA increased multinucleate cells, effect double was additive. induced reduction phosphorylation vimentin Ser72 but Ser71, myosin light chain (MLC) Ser19, binding subunit phosphatase (MBS) Ser852. In contrast, led MLC MBS Ser852, Ser71 Ser72. Cleavage ezrin/radixin/moesin (ERM) altered Aurora-B- and/or Rho-kinase/ROCK-depleted addition, C3 toxin B treatment abolish ERM cells attaining These results suggest regulate progression cytokinesis without communicating each other, there may exist novel kinase which furrow.
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