Ontogenetic pattern of thyroid hormone receptor expression in the human testis.

摘要: We studied the spatiotemporal distribution of thyroid hormone nuclear receptors (TRs) α1 and α2 β messenger RNA (mRNA) levels in normal human testicular tissue during development adulthood. Nonpathological specimens from five aborted fetuses (17 23 weeks gestation, three two cases, respectively) four patients undergoing orchiectomy (18 months old 38-, 42-, 52-yr-old, were analyzed by Northern blot, semiquantitative RT-PCR amplification using DNA sequences or specifically designed primers for TR isoforms, situ hybridization. By PCR amplification, we found that TRα1 TRα2 are both expressed at different fetal adult testis. At all ages is higher levels. analysis showed hybridization signals corresponding to expression a ratio increased 2.6 17 gestation 12.0 In fact, dramatically decreased throughout development, bei...