Identification of a novel allele of peroxisome proliferator-activated receptor gamma (PPARG) and its association with resistance to Aeromonas salmonicida in Atlantic salmon (Salmo salar)

摘要: Bacterial and viral diseases are major problems in Atlantic salmon aquaculture, but may be challenged through selection of brood stock with enhanced survival to diseases. Today's strategy is based on controlled challenge tests using siblings the breeding candidates, thus indirect. Direct trait records candidates can potentially provided identification genetic variation linked susceptibility disease. Peroxisome proliferator-activated receptor gamma (PPARG) a lipid-sensing transcription factor primarily known for inducing fat-accumulation adipocytes, also lipid-accumulating macrophages, mammalian species. Here we report novel allele PPARG, pparg-2, salmon. pparg-2 has an insertion sixty nucleotides that encodes two additional copies almost perfect decapeptide motif, (F/C/Y)NHSPDR(S/N)HS, compared previously described pparg-1. pparg-1 contains six this repeat unit whereas eight present allele. mRNA was detectable kidney spleen random samples. Here, studied effect upon highly virulent bacterium, Aeromonas salmonicida, causing furunculosis, virus infectious anaemia (ISA), respectively, Norwegian aquaculture population ppar alleles were found significantly associated A. not ISA. better terms test rates being 0.32, 0.40 0.42 animals pparg-1,-1, pparg-1, -2 genotypes, respectively. We conclude linkage disequilibrium (LD) with, or identical to, locus contributing different furunculosis PPARG mapped group (LG8) could only positioned male map since all informative parents mapping families males. This first showing association between pparg immune response fish.