Comparison of several spectrofluorimetric methods for measuring RNA and DNA concentrations in the deposit-feeding bivalve Abra ovata
作者： A. Grémare , G. Vétion
DOI: 10.1016/0305-0491(94)90052-3
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摘要: Abstract DNA concentrations, RNA and corresponding RNA/DNA ratios of the deposit-feeding bivalve Abra ovata were measured using seven spectrofluorimetric methods. Highly significant differences in found. The analysis effects main steps involved these protocols showed that: (i) presence detergent extracting buffer is absolutely necessary; (ii) centrifugation step essential; (iii) there are several problems associated with purification nucleic acids phenol-chloroform-isoamylalcohol (this procedure affected linearity response DNA-specific fluorochrome, modified responses two non-specific fluorochromes; moreover, this effect was dependent on BSA concentration). Based results, a protocol for quantification recommended.
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sci-hub.se 参考文章(39)
J. L. Littlepage, M. J. Dagg, Relationships between growth rate and RNA, DNA, protein and dry weight in Artemia salina and Euchaeta elongata Marine Biology. ,vol. 17, pp. 162- 170 ,(1972) , 10.1007/BF00347307
F Hovenkamp, Growth differences in larval plaice Pleuronectes platessa in the Southern Bight of the North Sea as indicated by otolith increments and RNA/DNA ratios Marine Ecology Progress Series. ,vol. 58, pp. 205- 215 ,(1989) , 10.3354/MEPS058205
A. E. DeBevoise, G. L. Taghon, RNA:DNA ratios of the hydrothermal-vent vestimentiferans Ridgeia piscesae and R. phaeophiale indicate variations in growth rates over small spatial scales Marine Biology. ,vol. 97, pp. 421- 426 ,(1988) , 10.1007/BF00397772
Larry A. Bentle, Seshachalam Dutta, Jack Metcoff, The sequential enzymatic determination of DNA and RNA Analytical Biochemistry. ,vol. 116, pp. 5- 16 ,(1981) , 10.1016/0003-2697(81)90314-6
Alexandros Frantzis, Antoine Grémare, Gilles Vétion, Growth rates and RNA: DNA ratios in Paracentrotus lividus (Echinodermata: Echinoidea) fed on benthic macrophytes Journal of Experimental Marine Biology and Ecology. ,vol. 156, pp. 125- 138 ,(1992) , 10.1016/0022-0981(92)90020-B
Uwe Karsten, Albert Wollenberger, Determination of DNA and RNA in homogenized cells and tissues by surface fluorometry Analytical Biochemistry. ,vol. 46, pp. 135- 148 ,(1972) , 10.1016/0003-2697(72)90405-8
P Richard, J-P Bergeron, M Boulhic, R Galois, J Person-Le Ruyet, Effect of starvation on RNA, DNA and protein content of laboratory-reared larvae and juveniles of Solea solea Marine Ecology Progress Series. ,vol. 72, pp. 69- 77 ,(1991) , 10.3354/MEPS072069
CW Mordy, DJ Carlson, An evaluation of fluorescence technigues for measuring DNA and RNA in marine microorganisms Marine Ecology Progress Series. ,vol. 73, pp. 283- 293 ,(1991) , 10.3354/MEPS073283
Arnt J. Raae, Ingegjerd Opstad, Per Kvenseth, Bernt Th. Walther, RNA, DNA and protein during early development in feeding and starved cod (Gadus morhua L.) larvae Aquaculture. ,vol. 73, pp. 247- 259 ,(1988) , 10.1016/0044-8486(88)90059-2
C. M. Clemmesen, Laboratory studies on RNA/DNA ratios of starved and fed herring (Clupea harengus) and turbot (Scophthalmus maximus) larvae Ices Journal of Marine Science. ,vol. 43, pp. 122- 128 ,(1987) , 10.1093/ICESJMS/43.2.122