The antioxidant effects of garlic saponins protect PC12 cells from hypoxia-induced damage.

摘要: Hypoxia frequently occurs under several different cellular circumstances. Excess reactive oxygen species that are induced by hypoxia may result in cell injury and dysfunction. Recently, garlic has been found to possess some biological pharmacological activities. The present study examined the effects of saponins (GSP) on survival differentiated PC12 (dPC12) cells oxidative–antioxidant system. dPC12 were exposed 2 % O2 order establish a neuronal insult model. Cell viability was determined 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide reduction assay lactate dehydrogenase (LDH) release assay. expression selected genes (catalase (CAT), p65 neuron-specific class III β-tubulin) evaluated real-time PCR immunoblot assays. CAT activity, malondialdehyde (MDA) 8-hydroxy-deoxyguanosine (8-OH-dG) concentrations also determined. data showed dramatically damaged cells, while treatment with approximately 5 × 10− 2–10 ng/ml GSP improved viability, decreased LDH leakage caused maintain neuronal-like characteristics hypoxia. production MDA 8-OH-dG attenuated GSP. activity pretreated higher than hypoxic control. Moreover, up-regulated total protein as well nuclear cells. These indicate antioxidant properties can protect from hypoxia-induced damage, which be related up-regulation decrease nucleus distribution through redox-sensitive signalling pathways.