The apoptotic pathway in fertile and subfertile men: a case-control and prospective study to examine the impact of merocyanine 540 bodies on ejaculated spermatozoa.
作者： Nardhy Gomez-Lopez , Guadalupe Estrada-Gutierrez , Alinne Colin , Arturo Flores-Pliego , Xochitl Flores-Escobar
DOI: 10.1016/J.FERTNSTERT.2012.11.052
关键词:
摘要: Objective To evaluate the presence of merocyanine 540 (M540) bodies and their impact on measurement apoptotic biomarkers in human spermatozoa. Design Case-control, prospective study. Setting Academic centers. Patient(s) Fertile subfertile subjects. Intervention(s) Semen samples from fertile men, 11 per group, were analyzed for basic semen parameters early (annexin-V binding) late (terminal deoxynucleotidyl transferase dUTP nick end labeling [TUNEL]) sperm by flow cytometry. Samples also stained with M540 to assess bodies. Main Outcome Measure(s) Presence Result(s) Groups differed significantly expression apoptosis biomarkers. The percentage between groups was not different. exclusion TUNEL results did have a significant either or groups. Conclusion(s) This study confirmed occurrence that male factor infertility is associated an increased Moreover, we demonstrated affect quantification group.
fertstert.org
sci-hub.se 参考文章(43)
Thinus F. Kruger, Anibal A. Acosta, Kathryn F. Simmons, R. James Swanson, James F. Matta, Sergio Oehninger, Predictive value of abnormal sperm morphology in in vitro fertilization Fertility and Sterility. ,vol. 49, pp. 112- 117 ,(1988) , 10.1016/S0015-0282(16)59660-5
Sara Marchiani, Elisabetta Baldi, Beatrice Fuzzi, Gianni Forti, Sara Dabizzi, Monica Muratori, Lara Tamburino, Ivo Noci, Chiara Pucci, Luciana Criscuoli, Paolo Evangelisti, Biological meaning of ubiquitination and DNA fragmentation in human spermatozoa. International Symposium: Steroid Hormone Receptor Superfamily and Molecular Signaling. ,vol. 63, pp. 153- 158 ,(2007)
J P Twigg, P A Milne, E L Gordon, N Fulton, R J Aitken, D S Irvine, DNA Integrity in Human Spermatozoa: Relationships With Semen Quality Journal of Andrology. ,vol. 21, pp. 33- 44 ,(2000) , 10.1002/J.1939-4640.2000.TB03273.X
Monica Muratori, Ilaria Porazzi, Michaela Luconi, Sara Marchiani, Gianni Forti, Elisabetta Baldi, ANNEXIN V BINDING AND MEROCYANINE STAINING FAIL TO DETECT HUMAN SPERM CAPACITATION. Journal of Andrology. ,vol. 25, pp. 797- 810 ,(2004) , 10.1002/J.1939-4640.2004.TB02858.X
Marcello Spanò, Jens P Bonde, Henrik I Hjøllund, Henrik A Kolstad, Eugenia Cordelli, Giorgio Leter, Sperm chromatin damage impairs human fertility‡‡The Danish First Pregnancy Planner Study is a collaborative follow-up study on environmental and biological determinants of fertility. The project is coordinated by the Steno Institute of Public Health, Aarhus University and is undertaken in collaboration with the Department of Growth and Reproduction, National University Hospital, Copenhagen. The team includes Jens Peter E. Bonde, Niels Henrik I. Hjøllund, Tina Kold Jensen, Tine Brink Henriksen, Henrik A. Kolstad, Erik Ernst, Aleksander Giwercman, Niels Erik Skakkebæk, and Jørn Olsen. Fertility and Sterility. ,vol. 73, pp. 43- 50 ,(2000) , 10.1016/S0015-0282(99)00462-8
L L Stunz, D W Peckham, J K Fishbaugh, V A Illera, D A Mower, R F Ashman, Decreased membrane phospholipid packing and decreased cell size precede DNA cleavage in mature mouse B cell apoptosis. Journal of Immunology. ,vol. 152, pp. 4832- 4842 ,(1994)
Monica Muratori, Sara Marchiani, Mario Maggi, Gianni Forti, Elisabetta Baldi, None, Origin and biological significance of DNA fragmentation in human spermatozoa Frontiers in Bioscience. ,vol. 11, pp. 1491- 1499 ,(2006) , 10.2741/1898
Sergey I. Moskovtsev, Jennifer Willis, John White, J. Brendan M. Mullen, Sperm DNA damage: correlation to severity of semen abnormalities. Urology. ,vol. 74, pp. 789- 793 ,(2009) , 10.1016/J.UROLOGY.2009.05.043
Rahul Rathi, Ben Colenbrander, Mart M. Bevers, Barend M. Gadella, Evaluation of In Vitro Capacitation of Stallion Spermatozoa Biology of Reproduction. ,vol. 65, pp. 462- 470 ,(2001) , 10.1095/BIOLREPROD65.2.462
S-C Ng, A Bongso, SS Ratnam, Microinjection of human oocytes: A technique for severe oligoasthenoteratozoospermia International Journal of Gynecology & Obstetrics. ,vol. 38, pp. 339- 339 ,(1992) , 10.1016/0020-7292(92)91063-T