Synthesis and assembly of adenovirus 2

摘要: Abstract Adenovirus type 2 hexon polypeptides have been traced from their synthesis on cytoplasmic polyribosomes through incorporation into whole virions. Analysis of the methionine-containing tryptic peptides and penton revealed that they had no in common, eliminating possibility a precursor-product relationship. Nascent were found polysomes required 3–4 min to be synthesized released ribosomes. Between 80% 90% assembled complete capsomeres within an additional release ribosome, while remaining 10–20% more slowly. The base fiber ribosomal site approximately min. Although there was initial rapid association 24% pentons, many hours for completion this assembly. Studies morphogenesis virions showed radioactive core began appear 15 synthesis, when still undetectable virus.