CHANGES IN THE RIBOSOME DISTRIBUTION DURING INCUBATION OF RABBIT RETICULOCYTES IN VITRO.
作者： Paul M. Knopf , Hildegard Lamfrom
DOI: 10.1016/0005-2787(65)90186-3
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摘要: Abstract Intact reticulocytes incubated in vitro synthesize hemoglobin at a linear rate for 1 h 30°. During the first 30 min about one-third of 80-S ribosomes are transferred into polysome fraction, leading to an increase concentration and size polysomes. Protein synthesis is accompanied by breakdown, this dominant event. These changes ribosome distribution appear be result initiation process (attachment polysomes) rather than rates peptide bond formation or release completed polypeptide chains from ribosomes. The rate-limiting step protein was located by: (a) determining synthetic time β-chain within two different intervals during incubation, (b) investigating kinetics under conditions where amino acids were limiting.
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sci-hub.se 参考文章(11)
Henry Borsook, Clara L. Deasy, A.J. Haagen-Smit, Geoffrey Keighley, Peter H. Lowy, INCORPORATION IN VITRO OF LABELED AMINO ACIDS INTO PROTEINS OF RABBIT RETICULOCYTES Journal of Biological Chemistry. ,vol. 196, pp. 669- 694 ,(1952) , 10.1016/S0021-9258(19)52400-0
A.P. Mathias, R. Williamson, H.E. Huxley, S. Page, Occurrence and function of polysomes in rabbit reticulocytes Journal of Molecular Biology. ,vol. 9, pp. 154- IN11 ,(1964) , 10.1016/S0022-2836(64)80097-8
HOWARD M. GOODMAN, ALEXANDER RICH, Mechanism of Polyribosome Action During Protein Synthesis Nature. ,vol. 199, pp. 318- 322 ,(1963) , 10.1038/199318A0
Alfred Gierer, Function of aggregated reticulocyte ribosomes in protein synthesis Journal of Molecular Biology. ,vol. 6, pp. 148- IN8 ,(1963) , 10.1016/S0022-2836(63)80131-X
Jerry B. Lingrel, Henry Borsook, A Comparison of Amino Acid Incorporation into the Hemoglobin and Ribosomes of Marrow Erythroid Cells and Circulating Reticulocytes of Severely Anemic Rabbits Biochemistry. ,vol. 2, pp. 309- 314 ,(1963) , 10.1021/BI00902A022
F. O. WETTSTEIN, T. STAEHELIN, H. NOLL, Ribosomal Aggregate Engaged in Protein Synthesis: Characterization of the Ergosome Nature. ,vol. 197, pp. 430- 435 ,(1963) , 10.1038/197430A0
HANS NOLL, THEOPHIL STAEHELIN, F. O. WETTSTEIN, Ribosomal Aggregates Engaged in Protein Synthesis : Ergosome Breakdown and Messenger Ribonucleic Acid Transport Nature. ,vol. 198, pp. 632- 638 ,(1963) , 10.1038/198632A0
Hildegard Lamfrom, Paul M. Knopf, INITIATION OF HAEMOGLOBIN SYNTHESIS IN CELL-FREE SYSTEMS. Journal of Molecular Biology. ,vol. 9, pp. 558- 575 ,(1964) , 10.1016/S0022-2836(64)80227-8
Hildegard Lamfrom, Factors determining the specificity of hemoglobin synthesized in a cell-free system Journal of Molecular Biology. ,vol. 3, pp. 241- 252 ,(1961) , 10.1016/S0022-2836(61)80064-8
Henry Borsook, Edmond H. Fischer, Geoffrey Keighley, Factors affecting protein synthesis in vitro in rabbit reticulocytes. Journal of Biological Chemistry. ,vol. 229, pp. 1059- 1070 ,(1957) , 10.1016/S0021-9258(19)63708-7