Activity of amphotericin B and itraconazole against intraphagocytic Candida albicans

摘要: The activity of amphotericin B and itraconazole against intracellularCandida albicans was determined in vitro using murine resident peritoneal macrophages. Amphotericin at concentrations 0.5 2 µg/ml produced significantly less rapid killing intracellular than extracellularCandida as measured macrophage-free medium. 0.1 or up to 3 only fungistatic limited fungicidal both extracellular organisms. Against acted by direct antifungal action rather through stimulation macrophage function, demonstrated the fact that (i) persisted when macrophages were successively exposed B, washed disrupted sonication, (ii) no seen tested B-resistantCandida tropicalis orSalmonella typhimurium. Pre-exposure (0.4 µg/ml) inhibited subsequent (2 susceptibleCandida albicans. These experiments validate conventional methods susceptibility testing for determining agents.