Histological and immunohistochemical evaluation of the chemopreventive role of lycopene in tongue carcinogenesis induced by 4-nitroquinoline-1-oxide

摘要: Abstract Objective Increasing attention has been given to the potential protective roles of specific antioxidant nutrients found in fruits and vegetables. However, there are relatively few reports on cancer chemopreventive effects lycopene or tomato carotenoids animal models. Therefore, efficacy with regard oral carcinogenesis was investigated using 4-nitroquinoline-1-oxide (4-NQO) induced tongue squamous cell carcinoma. Materials methods Twenty albino rats were equally divided into 2 groups. 4-NQO dissolved drinking water (20 ppm) for both Rats group administered at a dose 2.5 mg/kg body weight by intragastric intubation once day. Incidence neoplasms histopathological changes microscopically evaluated after 32 weeks administration treatments. Moreover, immunohistochemical expressions proliferating nuclear antigen (PCNA), E-cadherin β-catenin analysed specimens an image analyser computer system. Results Lycopene treatment significantly decreased incidence carcinogenesis. A percentage PCNA-positive nuclei associated treatment. Proliferating cells mainly confined basal parabasal epithelial layers. Increased immunoexpression recorded treated comparison carcinogen group. Conclusion present study indicate that can exert against through reduction proliferation enhanced cellular adhesion, suggesting new mechanism anti-invasive effect lycopene. Further studies needed provide more definitive answers question anticancer