Torque Teno Virus 10 Isolated by Genome Amplification Techniques from a Patient with Concomitant Chronic Lymphocytic Leukemia and Polycythemia Vera

摘要: An infectious etiology has been proposed for many human cancers, but rarely have specific agents identified. One difficulty the need to propagate cancer cells in vitro produce agent detectable quantity. We hypothesized that genome amplification from small numbers of could be adapted circumvent this difficulty. A patient with concomitant chronic lymphocytic leukemia (CLL) and polycythemia vera (PV) requiring therapeutic phlebotomy donated a large amount phlebotomized blood test possibility. Using methods, we identified new isolate (BIS8-17) torque teno virus (TTV) 10. The presence sequence 8–17 original plasma was confirmed by polymerase chain reaction (PCR), validating approach, since TTV is known virus. Subsequent PCR testing plasmas additional patients showed BIS8-17 had similar incidence (~20%) CLL (n = 48) or PV 10) compared healthy controls 52). do not harbor BIS8-17; did detect it peripheral genomic deoxyribonucleic acid (DNA) 20). clinical outcome prognostic markers (immunoglobulin heavy variable region [IGHV] mutation, CD38 zeta-chain associated protein kinase 70kDa [ZAP-70]) correlate infection. Although causative our knowledge, first reported isolation detection either PV. serve as covirus another variant rearranged genetic components contribute disease pathogenesis. These results prove method identifies provides an experimental methodology correlation disease.