Effects of soil organic matter and bacterial community shift on bioremediation of diesel-contaminated soil

摘要: Abstract Bioremediation of diesel-contaminated soils were applied to investigating effects soil organic matter (SOM) and bacterial community shift. Soil samples artificially contaminated with diesel oil, ranging from 4000 12000 mg/kg soil, remediated laboratory-scale landfarming batch applications. The SOM levels in our experiment 2.3% (presented as SOM15), 8.9% (SOM092), 11.8% (SOM125). Based on each the levels, bioremediation approaches bioaugmentation (BA015, BA092, BA125) using indigenous microorganisms control groups (CT015, CT092, CT125) tested. After about 300-day operation, total petroleum hydrocarbon (TPH) degradation efficiency became 73%, 63%, 59% SOM015, SOM092, SOM125, respectively. Their 1st order rates also reduced increase SOM. We preliminarily concluded that affected TPH rates. With a logarithm transformation, pattern SOM092 SOM125 found resemble other. No apparent improvement was BA batches. Our Intergenic spacer (ITS) microarray result indicated existence diesel-degrading bacteria communities. Nonmetric multidimensional scaling (MDS) based terminal restriction fragment length polymorphism (T-RFLP) data 1) CT similar community, once stage started, impling an activation bacteria; 2) dynamics more than or remediation could do, 3) both BA092 BA125 located same cluster MDS plot all time, revealing communities might cause comparable patterns SOM125. shift useful explaining performance.