Molecular characterisation of Czech Clostridium difficile isolates collected in 2013-2015

摘要: Clostridium difficile is a leading nosocomial pathogen and molecular typing crucial part of monitoring its occurrence spread. Over three-year period (2013-2015), clinical C. isolates from 32 Czech hospitals were collected for characterisation. Of 2201 isolates, 177 (8%) non-toxigenic, 2024 (92%) toxigenic (tcdA tcdB) these, 677 (33.5%) carried genes binary toxin production (cdtA, cdtB). Capillary-electrophoresis (CE) ribotyping the yielded 166 different CE-ribotyping profiles, which 53 represented by at least two each profile. 29 patterns common to Leeds-Leiden reference strain library WEBRIBO database (83.7% isolates), 24 recognized only (11.2% isolates). Isolates belonging these profiles comprised 94.9% all isolates. The ten most frequent 176 (n=588, 26.7%), 001 (n=456, 20.7%), 014 (n=176, 8%), 012 (n=127, 5.8%), 017 (n=85, 3.9%), 020 (n=68, 3.1%), 596 (n=55, 2.5%), 002-like (n=45, 2.1%), 010 (n=35, 1.6%) 078 (n=34, 1.6%). Multi-locus sequence (MLST) seven housekeeping performed in one isolate revealed 40 types (STs). We conclude that characterisation high diversity profiles; prevailing RTs (20.7%) (027-like, 26.7%).