The emergence of Clostridium difficile PCR-ribotype 001 in Slovakia

摘要: The purpose of this study was to determine the incidence Clostridium difficile infections (CDI) and characterise isolates in 14 departments ten academic hospitals Slovakia. During a one-month (September 2012) all unformed stool samples were investigated using rapid test detect presence GDH toxins A/B. Positive cultured anaerobically C. characterised by ribotyping, multiple-locus variable-number tandem repeats analysis, gyrA, rpoB ermB investigation. A total 194 38 (19.6 %) had positive test. Of samples, 27 revealed result for free A/B stool, 11 only GDH. mean CDI 2012 5.2 cases per 10,000 patient bed-days. Twenty available molecular analysis; seventeen belonged PCR-ribotype 001 (85 %) whereas remaining three identified as PCR-ribotypes 017, 078 449. MLVA two clonal complexes close genetic relatedness between from six different hospitals. Molecular analysis antibiotic-resistance determinants gene encoding resistance MLSB group antibiotics 90 % isolates, Thr82Ile amino acid substitution gyrA associated with fluoroquinolones 85 % isolates. We conclude that is predominant Slovakia strong potential spread development multidrug resistance.