Needleless electrospun and centrifugal spun poly-ε-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs.

摘要: Abstract The biofunctionalization of scaffolds for tissue engineering is crucial to improve the results regenerative therapies. This study compared effect platelet-functionalization 2D electrospun and 3D centrifugal spun on osteogenic potential hMSCs. Scaffolds prepared from poly-e-caprolactone, using electrospinning spinning technology, were functionalized five different concentrations platelets. Cell proliferation, metabolic activity differentiation tested hMSCs cultured in differential non-differential medium. porous structure fibers resulted higher cell proliferation. Furthermore, functionalization with platelets a dose-dependent increase activity, proliferation production an marker – alkaline phosphatase. was further promoted by culture combination both media shows improved osteoinduction environments rich inorganic phosphate ascorbate. Nevertheless, showed that optimal concentration induction hMSC osteogenesis range 900–3000 × 109 platelets/L. determines adhered platelets, use bone engineering.