Low-intensity Far-Red Light Inhibits Early Lesions That Contribute to Diabetic Retinopathy: In Vivo and in Vitro

摘要: PURPOSE Treatment with light in the far-red to near-infrared region of spectrum (photobiomodulation [PBM]) has beneficial effects tissue injury. We investigated therapeutic efficacy 670-nm PBM rodent and cultured cell models diabetic retinopathy. METHODS Studies were conducted streptozotocin-induced rats retinal cells. Diabetes-induced abnormalities assessed functionally, biochemically, histologically vivo vitro. RESULTS observed on neural vascular elements retina. Daily treatment (6 J/cm(2)) resulted significant inhibition diabetes-induced death ganglion cells, as well a 50% improvement ERG amplitude (photopic b wave responses) (both P < 0.01). To explore mechanism for these effects, we examined physiologic molecular changes related survival, oxidative stress, inflammation. did not alter cytochrome oxidase activity retina or inhibited superoxide production preserved MnSOD expression vivo. Diabetes significantly increased both leukostasis ICAM-1, essentially prevented abnormalities. In 30-mM glucose exposure production, inflammatory biomarker expression, death. all CONCLUSIONS ameliorated lesions retinopathy reduced stress been documented have minimal risk. is noninvasive, inexpensive, easy administer. conclude that simple adjunct therapy attenuate development