Ochratoxigenic black species of Aspergilli in grape fruits of northern Italy identified by an improved PCR-RFLP procedure.
作者： Davide Spadaro , Subban Patharajan , Alessia Lorè , Angelo Garibaldi , Maria Lodovica Gullino
关键词:
摘要: A collection of 356 isolates Aspergillus spp. collected during 2006 and 2007 from grapevines in northern Italy were identified through Internal Transcribed Spacer based Restriction Fragment Length Polymorphism (ITS-RFLP) tested for ochratoxin (OTA) production. endonuclease digestion the ITS products using endonucleases HhaI, HinfI RsaI, distinguished five different RFLPs. From each pattern, three samples sequenced nucleotide sequences showed species corresponding to niger, A. carbonarius, tubingensis, japonicus aculeatus. By comparing regions, also uniseriate aculeatus could be differentiated by products. Among aspergilli, niger was major associated with grapes (57.4%), while carbonarius (46.6%). All strains their ability produce OTA on Yeast extract sucrose medium (YES), as it an optimal substrate evaluation production black aspergilli. Out isolates, 63 (17.7%) produced ranging 0.05 3.0 µg mL−1. Most ochratoxigenic (46) both years, but some tubingensis (11) (6) lower amounts OTA.
mdpi.com
sci-hub.se 参考文章(49)
M. Bau, M.R. Bragulat, M.L. Abarca, S. Minguez, F.J. Cabañes, Ochratoxigenic species from Spanish wine grapes. International Journal of Food Microbiology. ,vol. 98, pp. 125- 130 ,(2005) , 10.1016/J.IJFOODMICRO.2004.05.015
E. E. Wafa, E. W., Yahya, R. S., Sobh, M. A., Eraky, I., El-Baz, M., El-Gayar, H. A. M., Betbeder, A. M. and Creppy, Human ochratoxicosis and nephropathy in Egypt: A preliminary study Human & Experimental Toxicology. ,vol. 17, pp. 124- 129 ,(1998) , 10.1177/096032719801700207
János Varga, Ferenc Kevei, Aldwin Vriesema, Fons Debets, Zofia Kozakiewicz, James H. Croft, Mitochondrial DNA restriction fragment length polymorphisms in field isolates of the Aspergillus niger aggregate. Canadian Journal of Microbiology. ,vol. 40, pp. 612- 621 ,(1994) , 10.1139/M94-098
G. Perrone, A. Susca, G. Cozzi, K. Ehrlich, J. Varga, J.C. Frisvad, M. Meijer, P. Noonim, W. Mahakarnchanakul, R.A. Samson, Biodiversity of Aspergillus species in some important agricultural products Studies in Mycology. ,vol. 59, pp. 53- 66 ,(2007) , 10.3114/SIM.2007.59.07
D. Spadaro, A. Lorè, A. Garibaldi, M.L. Gullino, Occurrence of ochratoxin A before bottling in DOC and DOCG wines produced in Piedmont (Northern Italy) Food Control. ,vol. 21, pp. 1294- 1297 ,(2010) , 10.1016/J.FOODCONT.2010.02.017
Lucie Pařenicová, Pernille Skouboe, Jens Frisvad, Robert A. Samson, Lone Rossen, Marjon ten Hoor-Suykerbuyk, Jaap Visser, Combined Molecular and Biochemical Approach Identifies Aspergillus japonicus and Aspergillus aculeatus as Two Species Applied and Environmental Microbiology. ,vol. 67, pp. 521- 527 ,(2001) , 10.1128/AEM.67.2.521-527.2001
Margo A. Kusters-van Someren, Robert A. Samson, Jaap Visser, The use of RFLP analysis in classification of the black Aspergilli: reinterpretation of the Aspergillus niger aggregate Current Genetics. ,vol. 19, pp. 21- 26 ,(1991) , 10.1007/BF00362083
Angel Medina, Rufino Mateo, Laura López-Ocaña, Francisco Manuel Valle-Algarra, Misericordia Jiménez, Study of Spanish Grape Mycobiota and Ochratoxin A Production by Isolates of Aspergillus tubingensis and Other Members of Aspergillus Section Nigri Applied and Environmental Microbiology. ,vol. 71, pp. 4696- 4702 ,(2005) , 10.1128/AEM.71.8.4696-4702.2005
T.J. White, T. Bruns, S. Lee, J. Taylor, Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics PCR protocols: a guide to methods and applicationsPCR protocols: a guide to methods and applications. ,vol. 18, pp. 315- 322 ,(1990) , 10.1016/B978-0-12-372180-8.50042-1
Douglas Hanahan, Studies on transformation of Escherichia coli with plasmids Journal of Molecular Biology. ,vol. 166, pp. 557- 580 ,(1983) , 10.1016/S0022-2836(83)80284-8